凋亡抑制基因survivin在膀胱移行细胞癌中表达的初步研究

A novel antiapoptosis gene,survivin,expression in bladder transitional cell cancer

目的 :探讨新的凋亡抑制基因survivin在膀胱移行细胞癌 (bladdertransitionalcellcancer,BTCC)中的表达 ,及其与p5 3、bcl- 2和ki6 7基因表达的相关性。 方法 :应用免疫组织化学链霉抗生物素蛋白—过氧化物酶连接法 (SP法 ) ,检测sur vivin、bcl- 2、p5 3和ki6 7在 5 8例BTCC中的表达 ,另选 10例癌旁正常组织作对照。结果 :survivin基因在癌旁正常组织中无表达 ,而在BTCC中呈高表达 ,survivin的表达与BTCC的临床分期和病理分级密切相关。 5 8例BTCC中 ,4 1例表达阳性 ,占70 .7%。同时发现survivin的表达与 p5 3、bcl- 2、ki6 7的表达也密切相关。 结论 :survivin基因的过度表达单独或和 p5 3、bcl-2、ki6 7协同在BTCC的发生发展中起重要作用 ,其过度表达提示BTCC预后不良。survivin可能会成为膀胱移行细胞癌一个新的诊断指标和治疗的靶点。

Objective:To study the expression of a novel inhibitor gene of apoptosis,survivin,in bladder transitional cell cancer,and its relationship with the expression of p53,bcl-2 and ki67 genes.Methods:Using streptavidin-biotin peroxidase(SP) method,we examined the expression of survivin,p53,bcl-2 and ki67 proteins in 10 normal bladder tissues and 58 bladder transitional cell cancer tissues.Results:Survivin was expressed in 41 of 58(70.7%) cases of BTCC.In contrast,normal bladder epithelium did not express survivin.Overexpression of survivin was related to the tumor grade and clinical stage.The expression of survivin was strongly correlated with p53.bcl-2 and ki67 expression.Conclusion:Apoptosis inhibition by survivin, alone or in cooperation with p53 and bcl-2 may participate in the onset and progression of bladder transitional cell cancer.Survivin could be a new diagnostic/therapeutic target in bladder tranditional cell cancer.