柯萨奇B病毒对不同人胰岛β细胞表达CD56的影响

Effect of Coxsackie virus B infection on expression of CD56 in different human islet β cells

目的 :研究柯萨奇B病毒 (CVB)感染对 3种人胰岛 β细胞和正常人胰岛 β细胞 (NBC)表面CD5 6表达的影响。方法 :利用流式细胞仪技术分析病毒作用 3种人胰岛 β细胞CM、HP6 2、TRM6以及NBC前后细胞表面表达CD5 6的变化。结果 :病毒作用前不同人胰岛 β细胞CD5 6表达量不同 ,HP6 2细胞表达量最高 ,其次是NBC ,CM细胞表达量最低。CVB3和CVB4作用于CM细胞 2 4h促进细胞表面CD5 6的表达 ,作用 4 8h细胞表面CD5 6的表达量下降。CVB3和CVB4作用于HP6 2细胞、TRM6细胞、NBC 2 4h、4 8h细胞表面CD5 6的表达量降低。结论 :随着病毒作用人胰岛 β细胞时间的延长 ,细胞表面CD5 6表达量下降 ,CD5 6是否在IDDM致病中起作用有待进一步研究。

AIM: To study effect of Coxsackie virus B (CVB) on expression of CD56 of three kinds of human islet β cell lines (CM,HP62 and TRM6) and normal β cells (NBC). METHODS: Using flow cytometric analysis to identify change of expression of CD56 on CM cell line,HP62 cell line,TRM6 cell line and NBC. RESULTS: Before contact with CVB there was different CD56 expression among these human islet β cells. The highest was HP62 cell line,the lowest was CM cell line. CD56 of CM cell line increased 24 hours after contact with virus,decreased 48 hours later. HP62,TRM6,and NBC all decreased after contact with virus.CONCLUSION: Coxsackie virus B decreases CD56 expression in human islet β cells,role of CD56 in pathogenesis of insulin-dependent diabetes mellitus still need further study.