牛磺酸减轻β-甘油磷酸诱导的大鼠血管平滑肌细胞钙化

Taurine attenuated β-glycerophosphate-stimulated calcification in cultured rat vascular smooth muscle cells

目的 :观察牛磺酸对钙化的形成及逆转作用的影响 ,探讨牛磺酸在血管钙化发生中的作用。方法 :利用β -甘油磷酸制备钙化血管平滑肌细胞 (VSMCs) ,测定细胞钙含量、碱性磷酸酶活性、[45Ca]沉积及 [3 H] 胸腺嘧啶。结果 :与对照组相比 ,钙化细胞的钙含量、ALP活性和 [45Ca]沉积均明显升高 (P <0 0 5 ) ,而牛磺酸与 β -甘油磷酸同时孵育呈剂量依赖性地抑制钙化发生。在牛磺酸逆转实验中 ,钙化细胞的钙含量、ALP活性和 [45Ca]沉积均较换液前明显降低 (P <0 0 1) ;且牛磺酸呈剂量依赖性地逆转已钙化细胞。与对照组相比 ,钙化细胞的细胞数量和[3 H] TdR掺入量增加 (P <0 0 1) ,牛磺酸早期干预组显著抑制钙化细胞的增殖 ,但牛磺酸对已钙化的细胞 ,增殖抑制效应不明显。结论 :牛磺酸可抑制细胞钙化形成且能逆转已形成钙化。

AIM: To investigate the effect of taurine on calcification of vascular smooth muscle cells (VSMCs).METHODS: Calcified VSMCs of rat in vitro were induced by β-glycerophosphate. Cellular calcium content,alkaline phosphatase(ALP) activities and accumulation were measured. DNA synthesis were evaluated by -thymidine (-TdR) incorporation. RESULTS: Calcium content,ALP activities and uptake of calcified VSMCs stimulated by taurine (5-20 mmol/L) were greatly decreased in a concentration-dependent manner as compared with calcified group ( P<0.01 ). Taurine also inhibited the proliferation of calcified cells in a concentration-dependent manner. Cell counting, TdR incorporation of calcified cells stimulated by taurine were greatly decreased as compared with calcified VSMCs ( P<0.01 ). CONCLUSION: It was demonstrated that calcification of VSMCs may be alleviated by taurine.