间充质干细胞体外成骨及成脂肪分化中sortilin基因表达的研究

Expression of sortilin gene on osteogenic and adipogenic differentiation in mesenchymal stem cells

目的 :研究间充质干细胞 (MSC)体外成骨、成脂肪分化时sortilin基因表达变化。方法 :从骨髓中分离培养人间充质干细胞 ,流式细胞术鉴定其表型 ,加入成骨和成脂肪诱导剂 ,RT -PCR检测成骨标志骨桥蛋白 (Op)和成脂肪标志脂蛋白脂肪酶 (LpL)表达 ,半定量RT -PCR分析sortilin基因随诱导时间的表达变化。结果 :①体外分离培养的人间充质干细胞能向成骨细胞和脂肪细胞分化 ,分别表达Op和LpL。②在体外成骨分化过程中 ,sortilin基因表达第 1d开始上调 ,持续约 1周后恢复至原水平 ;在培养 3d时 ,sortilin基因表达明显上调 ,与未诱导组相比 ,差异明显 (P <0 0 1)。③体外成脂肪分化时 ,sortilin基因表达无明显的改变 (P >0 0 5 )。结论 :sortilin基因可能参与间充质干细胞成骨分化的调节 ,而与成脂肪分化无关 ,通过调控sortilin基因的表达 ,有可能为成骨障碍性疾病的治疗 ,提供新的思想和策略。

AIM: To characterize the gene expression of sortilin on adipogenic and osteogenic differentiation in mesenchymal stem cells (MSCs) in vitro and explore its significance.METHODS: MSCs derived from human bone marrow were isolated and cultured in vitro ,then were stimulated in osteogenic medium and adipogenic medium,respectively. Osteopontin and lipoprotein lipase were detected by RT-PCR. Sortilin expression was analyzed by semiquantitative RT-PCR. RESULTS: 1.MSCs displayed the potential of differentiation into osteoblast and adipocyte. 2.Sortilin was upregulated one day after osteogenic induction and remained upregulated for a week. The expression of sortilin was significant increased on day 3( P <0.01). 3. No significant changes of sortilin expression was found in adipogenic differentiation ( P >0.05).CONCLUSION: Sortilin may be useful to modulate the osteogenic differentiation and may not be necessary for adipocyte commitment in MSCs. The regulation of sortilin expression may provide new protocal and strategy for the treatment of osteoporosis and osteopenic disease.