摘要
为探讨牛血清中存在的HBsAg样蛋白的性质,给HBV的发病机制、治疗、预防等研究提供依据,我们采集93份牛血清,以SDS-PAGE、WesternBlot对血清中HBsAg样蛋白进行研究,发现其分子量约为27kDa;以SDS-PAGE分离牛血清中的HBsAg样蛋白,经皮下多点免疫小鼠,可产生与人HBV编码蛋白HBsAg反应的抗体。表明该27kDa蛋白可能存在与HBsAg相似的免疫学特性。
To investigate the characteristics of an HBsAg-like protein in the bovine serum, SDS-PAGE and Western Blot were applied for identification of the molecular weight of this protein. A specific band with the molecular weight of 27kDa was detected in SDS-PAGE and Western Blot analyses. BALB/c mice were then immunized subcutaneously by using the 27kDa protein isolated by SDS-PAGE from bovine sera. Antibodies specifically reacted with HBsAg produced by HBV was detected in the serum derived from the mice immunized with the 27kDa protein. Thus, a 27kDa protein with antigenicity similar to HBsAg produced by HBV was found and characterized immunologically in bovine sera.
出处
《中国病毒学》
CSCD
2004年第1期6-9,共4页
Virologica Sinica
基金
国家杰出青年科学基金(39925031)
国家重点基础研究发展计划(2001CB10006)
关键词
牛血清
鉴定
抗原性
乙型肝炎病毒
Bovine sera
Identification
HBsAg
Western Blot
Antigenicity
