一种食用菌提取物y3对烟草花叶病毒的钝化作用及其机制

Study on inactivation of Tobacco mosaic virus by y3 from Mushroom (Coprinus comatus)

测定了从食用菌毛头鬼伞(Coprinuscomatus)中提纯的y3蛋白对烟草花叶病毒(Tobaccomosaicvirus,TMV)的钝化作用,结果表明y3对TMV有较强的体外钝化作用,在心叶烟枯斑寄主上对TMV的抑制中浓度约为2.0靏/mL;y3在pH9.0时较稳定;TMV与y3混合后用RNase处理,测得侵染率为61.74%,比未用RNase处理的对照降低了38.26%,说明y3具有一定的体外脱衣壳作用;另外电镜观察发现y3可使部分TMV粒体发生裂解,变短。

A antiviral protein y3, from mushroom Coprinus comatus, could inactivate Tobacco mosaic virus (TMV) in vitro. The inhibition rate against is 50.0% when the concentration of y3 is 2.0靏/mL. When RNase was added to the mixture of TMV and y3, the infection rate of TMV was 61.74%, decreased by 38.26% in contrast with the control. It was also shown that y3 can break TMV particles when observed under electron microscope.