HPLC-荧光法测定人血浆中卡维地洛浓度及其药动学研究

HPLC-fluorescence Determination of Carvedilol in Human Plasma and Study on its Pharmacokinetics

目的 :建立测定人血浆中卡维地洛浓度的方法 ,用于研究卡维地洛片的药代动力学。方法 :血样经乙醚萃取 ,再以稀硫酸回提 ,采用HPLC 荧光法检测。以CromHypersilODSC1 8柱为固定相 ,甲醇 0 0 3mol/L磷酸二氢钾 (70 :3 0 )为流动相 ,激发波长 2 85nm ,发射波长 3 4 0nm ,内标为 β 萘酚。 结果 :该法在 1 56～ 10 0ng/ml浓度范围内呈线性关系 ,r=0 9988。最低检测浓度 1 56ng/ml,平均提取回收率为 76 49% ,日内RSD 2 76%、日间RSD 4 67%。结论 :本法重现性好、灵敏度高 ,适用于临床药代动力学分析 。

AIM:A reversed-phase HPLC-f luorescen ce method was developed for the determination of carvedilol in human plasma and for the study of its pharmacokinetics in young volunteers METHOD: Carvedilol in p lasma was extracted with diethyl ether and re-extracted with sulphuric acid C hr omatography was performed on a Crom Hypersil ODS column(5 μm,250 mm×46 mm) wit h methanol-003 mol/L KH 2PO 4 (70∶30) as mobile phase, and β-nap h thol was used as internal standard The excitation and emission wavelength wa s 285 nm and 340 nm respectively RESULT:The regression line was linear within the concentration range of 156～100 μg/L(r=09988),and the limit of detec tion was 1 μg/L The extraction recovery was 7649%,and the RSD of within- day and betwee n-day were 276% and 467%,respectively CONCLUSION:The esta blish ed HPLC method was sensitive,accurate and precise. No endogenous interferenc e was found in chromatograms of the biological samples,and it was applied to the pharmacokinetics studies of carvedilol in human