肺腺癌酪氨酸激酶信号传导通路中基因异常表达的复杂性

The Complexity of the Abnormal Tyrosine Kinase Signaling Pathway in Lung Adenocarcinoma

目的 探讨肺腺癌患者酪氨酸激酶信号传导通路的紊乱情况。方法 抽提 10例肺腺癌标本及其癌旁组织的总RNA ,标记荧光 ,与含 13 82 4个基因的基因芯片进行杂交 ,经洗片、扫描 ,计算机分析比较两种组织的差异表达基因。每次实验重复两次 ,结果取平均值 ,共进行 2 0次杂交 ,最后统计分析酪氨酸激酶信号传导通路中各种组分的表达情况。结果 在酪氨酸激酶信号传导通路中 ,5例以上标本差异表达的基因共 67个 ,其中上调基因 3 1个 ,下调基因 3 6个 ,涉及配体、受体、相关酶类、转录因子及调控基因等。结论 肺腺癌患者酪氨酸激酶信号传导通路的异常是多个环节的 ,多种组分在转录水平上发生了不同程度的改变 ;生长因子与酪氨酸激酶介导的信号传导有不同程度的激活 ,而抗增殖和免疫防御的信号传导受到不同程度的抑制 。

Objective To study the abnormity of tyrosine kinase signaling pathway in lung adenocarcinoma. Methods Total RNA was extracted form 10 lung adenocarcinoma samples and matched normal tissues. cDNA was labeled fluorescence during reverse transcription and hybridized on 20 slides of microarray with 13824 genes. After washed and scanned, the cy3∶cy5 ratios were obtained by computer analysis. Each experiment was repeated two times and the mean value was chosen. At last we analysis the expression of many components in tyrosine kinase signaling pathway. Results In tyrosine kinase signaling pathway, there are 67 genes changed in over 5 samples, which 31 were up expressed and 36 were down, including ligand, receptor, enzyme, transcription factor, regulation genes and so on.Conclusion Gene expression profiles showed the abnormal tyrosine kinase signaling pathway in many steps and many components were changed. Growth factor and tyrosine kinase mediated signaling is activated and the signaling against cell proliferation is inhibited in some extent, which show a state of system complexity.