卵泡刺激素和雌激素对培养的鸡胚卵巢生殖细胞增殖的影响

Effects of Follicle-stimulating Hormone and Estrogen on Development of Embryonic Chicken Germ Cell in Vitro

在动物体内多种激素共同调控着卵巢生殖细胞的生长、发育和成熟 ,其中促性腺激素起着至关重要的作用。本实验建立了鸡胚 ( 1 8日胚龄 )卵巢构建生殖细胞和体细胞的共培养模型 ,并研究卵泡刺激素(FSH)、 1 7β雌二醇 (E2 )以及二者联合处理对生殖细胞增殖的影响。结果发现FSH ( 0 2 5～ 1 0IU/mL)、E2( 1 0～ 1 0 0 0ng/mL)处理 4 8h后均可显著促进生殖细胞的增殖 ,FSH与E2 共同处理作用更加显著且高于其单独处理组 ,表明FSH和E2 可协同刺激鸡胚卵巢生殖细胞的增殖 ,FSH可能是通过促进雌激素或其受体的合成而发挥作用。

The proliferation,development and maturation of ovarian germ cells are regulated by multiple hormones,among which gonadotropins play a key role.In this study a germ-somatic cell coculture model of 18-day-old chicken embryo ovarian cells was established to evaluate effects of follicle-stimulating hormone (FSH) and 17β-estradiol (E 2) alone or in combination on germ cell proliferation.Results showed that FSH (0.25-1IU/mL) and E 2(10-1000ng/mL) both showed an notable increment in germ cells number after 48h treatment.Furthermore,FSH manifested a synergistic effect with E 2 in stimulating germ cell proliferation.These results indicated that FSH might interact with estrogen to promote embryonic ovarian germ cell proliferation.FSH might exert its action by stimulating the synthesis of estrogen or expression of its receptors.