摘要
采用ABC免疫组织化学法 ,应用 5 -羟色胺 ( 5 HT)特异性抗血清 ,对枕纹锦蛇 (Elaphedione)消化道内含有的 5 HT内分泌细胞进行了免疫组织化学的定位研究和形态学观察。结果显示 ,5 HT细胞在消化道各部位的分布密度呈倒“V”形 ,以十二指肠最高 ,胃贲门部最低。其形态多样 ,上段 (食管、胃 )多为圆形和椭圆形 ,主要分布于上皮基部和腺泡上皮之间 ;中段 (十二指肠、空肠、回肠 )以细长锥体形、梭形、圆形为主 ,主要分布于上皮基部和上皮细胞之间 ;下段 (直肠 )为圆形 ,分布于上皮基部。锥体形细胞常有一个长突起伸入到固有膜或肠腔 ,行使内或外分泌功能 ;梭形细胞有两个细长突起 ,一个指向固有膜 ,另一个指向肠腔 ,表明这种细胞可能具有内、外分泌的双重功能。
The presence investigation and morphology observation of 5-HT endocrine cells have been carried out with the anti-5-HT serum by the method of the ABC immunhistochemical technique in the digestive tract of Elaphe dione.The results indicate that the distributive density of 5-HT cells is maximum in the duodenum and minimum in the cardiac part of stomach,and the density curve of 5-HT cells in gastrointestinal tract appears reverse “V”.The shape of 5-HT endocrine cells is diverse.The cells in superior segment (esophagus,and stomach) are round or ellipse,and disperse the bottom of epithelial or between gland epithelial cells mainly;those in middle segment (duodenum,jejunum,and ileum) are tapering,shuttle and round,and disperse the bottom of epithelial or between epithelial cells mainly;those in inferior segment (rectum) are round and disperse the bottom of epithelial cells.The tapering cells always have one long processus extending to either the gut lumen or lamina propria,and perform exocrine or endocrine function;the shuttle cells have two long processuses,one extending to gut lumen,the other extending to lamin propria,and perform both exocrine and endocrine functions.
基金
哈尔滨师范大学自然科学基金资助项目
蔡火石生物科学发展基金资助项目
