红花黄色素对衰老模型小鼠肝线粒体膜磷脂影响的实验研究

Experimental study of safflower yellow pigment on Phospholipid of hepatocytic mitochondria membrane in senile model mice

目的 :探讨小鼠肝线粒体膜磷脂组成的随龄变化及红花黄色素对其的影响。方法 :采用HPLC法测定线粒体膜PC、PE、CL的含量 ,陈思峰法测定PLA2 活性。结果 :衰老模型组PC、PE、CL均明显降低 ;PLA2 活性随增龄而升高。SYP可升高衰老模型组PC、PE、CL含量 ;显著降低衰老模型组PLA2 活性。结论 :D -半乳糖模型出现拟衰老时线粒体膜磷脂改变特征 ,SYP可显著改善肝线粒体膜磷脂的组成。

Objective:To investigate the age-related changes of phospholipid contents in hepatocytic mitochondria membrane of mice and the effect of safflower yellow pigment(SYP)on hepatocytic mitochondria.Methods:We tested the levels of hepatocytic mitochondria phosphatidyl chorine(PC),phosphatidyl ethanolamine (PE) and cardiolipin (CL) by high pressure liquid chromatography(HPLC) method;Activities of phospholipase A 2(PLA 2) by Chen Sifeng's method.Results:The levels of PC,PE and CL of senile model group were obviously descended,The Activities of PLA 2 was ascended by ageing in mitochondria( P <0.05);SYP cna enhance the levels of PC,PE and CL in mitochondria( P < 0.01 );But obviously descend the activities of PLA 2 of senile model mice.Conclusion:SYP can obviously improve the phospholipid contents in hepatocytic mitochondria membrane.