复方天麻口服液对偏头痛小鼠的作用

Effect of the Compound Tianma Oral Solution on the Migraine Model in Mice

目的 :观察复方天麻口服液对偏头痛小鼠的作用。方法 :昆明种小鼠 90只 ,随机分为 6组 ,每组 15只。第 1组为假手术组 ,皮下注射等体积的 0 .9%氯化钠注射液 ;第 6组为正常对照组 ,不做皮下注射 ;第 2～ 5组制成偏头痛模型 (小鼠皮下注射利血平 10mg·kg 1 ,14d ,并脑室注射血凝块 2 μg)后 ,第 2组给予 0 .9%氯化钠注射液 ,第 3～ 5组分别给予复方天麻口服液 2 .2 5 ,4.5 0 ,9.0 0g·kg 1 ,均灌胃给药 3d。采用热板法和扭体法测痛阈 ,取血测凝血时间 ,取脑匀浆测神经递质含量。结果 :复方天麻口服液能延长凝血时间 ,提高痛阈 ,调节 5 羟色胺 ( 5 HT)的过度降低 ,抑制脑内炎性刺激物的升高。结论

Objective:To survey the effect of compound tianma oral solution on the migrane model in mice. Methods:90 mice of the kunming species were randomly divided into 6 groups, with 15 animals in each. Mice of group 1 underwent sham operation. Those of group 6 served as normal control. A migraine model was set up for each mouse of group 2 to group 5 as follows. The animals were given each a daily hypodermic injection of 10 mg·kg -1 of reserpine for 14 consecutive days as well as an injection of 2 μL of blood clot into the cerebral ventricles. Thereafter, gastrogavage was used to administer 0.9% sodium chloride injection to each animal of group 2, 2.25, 4.50, 9.00 g·kg -1 of compound tianma oral solution to each of the mice of group 3, group 4 and group 5, respectively, for 3 consecutive days. Pain threshold was determined by the hot plate method and the writhing method. Blood was taken for the determination of coagulation time while brain homogenate was prepared for the assay of 5-HT, 5-HIAA and histamine. Results:Compound tianma oral solution was shown to prolong coagulation time, elevate pain threshold, prevent excessive decrease in 5-HT and inhibit the accumulation of inflammatory stimulants in the brain. Conclusion:The compound tianma oral solution was shown to be effective in the experimental treatment of migraine model in mice induced by reserpine and injection of blood clots into the cerebral ventricles.