镉对小鼠肾脏细胞凋亡的影响

Effect of cadmium on apoptosis of renal cells in mice

目的 研究镉对小鼠肾脏细胞凋亡的影响。方法 将小鼠分成 5组 ,分别注射 0 9%生理盐水 ,80、 160、2 40或 3 2 0mg/kg的CdCl2 溶液 ,连续 6周 ,于染毒 4周和 6周后每组处死一半小鼠 ,测定肾脏系数 ,用流式细胞术检测细胞凋亡 ,用免疫组化法测Bcl 2。结果 染镉组小鼠肾脏系数较对照组显著增高 ,而且细胞凋亡率较对照组明显增高 ,Bcl 2蛋白表达明显下降。

Objective To study the effects of cadmium on apoptosis of renal cells in mice.Method Eighty mice were randomly divided into five groups,and each group was injected intraperitoneally(ip)with 80,160,240,320 mg/kg of CdCl 2 and 0.9% NaCl per day for five days a week in 6 weeks.Half of the mice in each group were sacrificed 4 weeks after injection,and another half were sacrificed 6 weeks after injection.Kidney coefficients were measured and renal cell apoptosis was determined with flow cytometry.Expression of Bcl-2 protein was measured by immunohistochemistry.Result Kidney coefficients and apoptotic rates of renal cells in cadmium-treated mice were significantly higher than in controls.Expression of Bcl-2 protein decreased in cadmium-treated mice significantly.Conclusion Cadmium could induce apoptosis of renal cells in mice.