缬沙坦对压力超负荷下兔左室心肌TNF-α表达及胶原重构的影响

Effect of Valsartan on TNF-α expression and collagen remodeling in left ventricle of rabbit undergoing pressure overload

目的 :观察缬沙坦 (Valsartan)对慢性压力超负荷下左室心肌 TNF-α表达、胶原重构及心功能的影响。方法 :家兔 3 0只随机分为假手术组、腹主动脉次全结扎组 (简称结扎组 )、缬沙坦干预结扎组 (简称干预组 ) ,每组 10只。术后 12周进行血流动力学检查并取左室心肌行 VG染色及免疫组化染色分别观察胶原容积百分比 (CVF)及TNF-α表达情况。结果 :结扎组与假手术组比较 ,TNF-α表达显著增强 (P<0 .0 1) ,左室质量 /体质量 (L VW/BW)、左室舒张末压 (L VEDP)、含小血管视野的 CVF(CVF-V)及不含小血管视野的 CVF(CVF-NV)均显著升高 (均 P<0 .0 1) ;缬沙坦干预组与结扎组比较 ,TNF-α表达显著减弱 (P<0 .0 1) ,L VW/BW、L VEDP、CVF-V,CVF-NV显著降低 (均 P<0 .0 1)。结论 :缬沙坦可抑制压力负荷下心室肌 TNF-α的过度表达并减轻心室肌的胶原增生。

AIM:To study the effect of Valsartan on TNF-α expressio n and collagen remodeling in the left ventricle of the rabbit undergoing chronic pressure overl oad. METHODS:Thirty rabbits were randomly devided into 3 groups(n=10), as t he sham ope ration group, the abdominal aorta banded group(banded group), and the abdominal aorta banded +Valsartan group(Valsartan group). Twelve weeks after the operation , hemodynamic par a meters were acquired, then collagen volume fraction (CVF)and TNF-α expression o f the left ventricle were measured by using VG and immunohistochemical staining. RESULTS:Compared with the sham operation group, TNF-α expression was enh anced and LV W/BW, LVEDP, CVF-V,CVF-NV decreased significantly in the banded group(P <0.01). Compared with the banded group, TNF-α expression was weakened and LVW/BW, LVED P, CVF -V,CVF-NV increased significantly in the Valsartan group(P<0 01).CONCLUSION: Va lsartan can inhibit abnormal TNF-α expression, collagen proliferation, ventric ul e remodeling in left ventricle of rabbit undergoing chronic pressure overload,th us preserving heart function.