羟乙基淀粉与明胶对人体中性粒细胞和单核细胞吞噬作用的影响

Effects of hydroxyethyl starch and gelatine on phagocytic activity of human neutrophils and monocytes

目的 观察明胶和6％羟乙基淀粉200/0.5溶液对中性粒细胞和单核细胞吞噬作用的影响。方法 33例ASA Ⅰ-Ⅱ级的泌尿外科病人随机分为3组,组I:明胶组(n=11);组Ⅱ:羟乙基淀粉组(n=11);组Ⅲ:乳酸林格氏液(Bss)组(n=11)。每组病人均输注胶体或Bss 10 ml·kg-1,输注前及输注后1 h抽取静脉血,使用流式细胞仪测定中性粒细胞和单核细胞对异硫氢酸荧光素(FITC)标记的经调理的大肠杆菌的摄取数。结果 组Ⅰ输液后产生吞噬作用的中性粒细胞百分数显著性下降(P<0.05);单核细胞百分数显著性下降(P<0.01)。组Ⅱ、组Ⅲ输液前后无明显变化。结论 羟乙基淀粉对中性粒细胞和单核细胞的吞噬作用无明显影响,而明胶则使之减弱。

Objective To assess the effects of gelatine and 6% hydroxyethyl starch 200/0.5 (HES 200 / 0.5) on phagocytic activity of human neutrophils and monocytes using flow cytometry.Methods Thirty-three ASA Ⅰ - Ⅱ patients aged 18-70 years scheduled for urological minor surgery were randomly divided into three equal groups of eleven patients :group I gelatine;group II HES 200 / 0.5 and group Ⅲ lactated Ringer's solution (LR) . 10 ml·kg-1 of gelatine, HES or LR was infused over 60 min and venous blood samples were taken before infusion and 1 h after the start of infusion for determination of phagocytes with ingested FITC-labeled E coli by flow cytometry. Results In gelatine group the percentage of neutrophils and monocytes with phagocytic activity decreased significantly after infusion ( P < 0.05 or 0.01). There was no significant change in phagocytic activity of neutrophils and monocytes after infusion in HES and LR groups.Conclusion HES 200 / 0.5 has no significant effect on phagocytic activity of neutrophils and monocytes while gelatine decreases it.