牛血孢子虫单一种的分离技术及其保藏方法的研究——卵形巴贝斯虫的传播试验

STUDIES ON ISOLATION AND PRESERVATION OF A SINGLE SPECIES OF HAEMATOCYTOZOON IN BOVINE——EXPERIMENTS ON TNE TRANSMISSION OF BABESIA OVATA

将实验室培育的“洁净”长角血蜱的幼虫、若虫、成虫及微小牛蜱的幼虫,先后分别释放到人工感染卵形巴贝斯虫单一种牛体上不同部位事先粘贴好的布袋中,使其自行叮咬吸血。待饱血脱落后亦分别收集,置28℃、相对湿度约90％的条件下蜕皮或产卵孵化。而后用不同世代和各变态期的蜱,分别叮咬感染除脾和非除脾健康易感牛。结果表明,长角血蜱的当代若虫和成虫对卵形巴贝斯虫没有传播能力,幼虫和若虫吸入的病原亦不能经卵传递;饱血雌虫吸入的病原可经卵传递,次代幼虫、若虫和成虫三个阶段都具有传播能力。次代感染幼虫经兔体后的若虫和成虫也具有感染力。 微小牛蜱不能传播该种病原。

An experiment on Haemaphysalis longicornis as a vector in transmission of Babesia ovata was made. The results showed that infection can be initiated only in the adult stage of the tick. Whilst larvae, nymphs and adults stages of second generation of an infected female can transmit the pathogen. The parasite taken in the larvae and nymphs could not be transmitted by first generation nymphs and adults moulted from them. The parasite taken in the stages can not also be transovarially transmitted. The nymphs and the adults developed from infected larvae which had passed through rabbit were able to transmit the pathogen. Boophilus microplus can not be a vector of transmitting Babesia ovata.