摘要
本文首次成功地建立了间接法Dot-ELISA检测新城疫病毒,对新城疫发病鸡群口咽、泄殖腔拭子的NDV抗原阳性检出率分别为58.33％(91/156)、62.50％(90/144),两者没有显著差异(P>0.05),结果表明,间接法Dot-ELISA对新城疫的早期诊断具有简单、快速、经济、敏感性高、特异性强和重复性好等优点,是疫病早期诊断与流行病学调查的有效手段。
It's the first time that the standardized procedure of indirect Dot-ELISA has been established for the detection of ND viral antigens.The frequency of ND viral antigen-positive oropharyngeal and cloacal swabs, collected from ND natural field outbreaks, was 58.33% (91/156) and 62.50% ( 90/144), respectively. There is no statistically significant difference between the oropharyngeal and cloacal swabs.
This study indicates that the indirect Dot-ELISA is an economical, reproduible, quick, easy-to-perform, sensitive and specific assay that can be used for early diagnosis of ND.It's an extremely efficient tool in the diagnosis and epidemiological investigations of diseases.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
1992年第2期187-192,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
关键词
间接法
新城疫
病毒
检测
Dot-ELISA, Indirect Dot-ELISA, NDV
