人钠/碘同向转运体基因cDNA的克隆与序列测定

Cloning and sequencing of human sodium/iodide symporter

为研究人钠/碘同向转运体(hNIS)的生物学性能和用于肿瘤放射性碘治疗的可能性,运用反转录聚合酶链反应 (RT-PCR)从人甲状腺组织总RNA中扩增出hNIS基因cDNA序列,将其克隆至pUCm-T载体中。序列分析证实克隆 片段与文献报道的hNIS基因cDNA序列完全一致,说明已成功克隆到hNIS基因cDNA。

For studying biological actions and potential utility as a radioiodide treatment for undifferentiated thyroid carcinoma and nonthyroid carcinoma, human sodium/iodide symporter (hNIS) gene cDNA was amplified with total RNA from human thyroid tissue by RT-PCR. The recombinant plasmid pUCm-T-NIS was constructed by inserting the hNIS cDNA into cloning vector pUCm-T and transformed into E.coli XL-B. Screen the positive clone which contains the cDNA of hNIS. The cloned hNIS cDNA sequence is identical to published sequence.