感染小鼠组织中Q热立克次体的分子病理学检测

Molecular pathological detection of Coxiella burnetii in infected BALB/c mice

腹腔注射Q热立克次体悬液感染BALB/c小鼠,发病后解剖,取主要组织脏器,应用免疫组化和原位杂交检测感染小鼠体内Q热立克次体的抗原分布和DNA在靶细胞中的表达,探讨Q热病变规律及致病机理。结果显示阳性信号多位于单核-巨噬细胞系统细胞胞浆内。结果提示免疫组化和原位杂交技术可以作为Q热特异性的诊断方法,并为致病机理的研究提供线索。

Immunohistochemical staining and in situ hybridization were applied to study the pathogenesis of Q fever and detect the antigen and DNA of Coxiella burnetii in BALB/c mice introperitoneally inoculated with Coxiella bur-netii. The positive signals of were all located at cytoplasm of monocyte-macrophages system. It indicates that im-munohistochemistry and in situ hybridization can provide reliable basis in diagnosis and pathological mechanism study of Q fever.