摘要
建立一种简便、快速、特异的PCR检测方法,用于鼠疫耶尔森氏菌的快速鉴定。针对鼠疫耶尔森氏菌特异的一段染色体序列3a设计引物,扩增一276 bp片段的鼠疫标识序列。应用该PCR反应体系,对我国17个生态型及1个待定的生态型共计275株鼠疫耶尔森氏菌及48株相关菌株的PCR扩增结果表明,实验菌株均扩增出预期的276 bp片段产物带,48株相关菌株均阴性,其检测灵敏度为100 pg DNA。说明该方法用于鼠疫耶尔森氏菌的检测鉴定简便、快捷,具有很高的特异性和敏感性。
To develop a simple and specific PCR method for rapid identification of Yersinia pestis strains. The PCR assay was based upon primers derived from Y. pestis highly specific region on chromosome to amplify a 276bp product to identify Y. pestis. The selected primers amplified a 276-bp product from all 275 Y. pestis strains tested. No PCR product was generated for closely related bacteria, including Y. pseudotuberculosis, Y. enterocolitica and others in Enterobacteriaceae. This PCR assay was rapid and specific for rapid identification of Y. pestis. it will be useful to identify Y. pestis in case of emergency and for the surveillance of epidemics.
出处
《生物技术通讯》
CAS
2003年第6期569-572,共4页
Letters in Biotechnology
