HPLC法检查小剂量阿司匹林肠溶片中的游离水杨酸

Test for free salicylic acid in enteric-coated tablets of small dose of aspirin

目的:建立HPLC法检查小剂量阿司匹林肠溶片中游离水杨酸的方法。方法:以十八烷基硅烷键合硅胶为填充剂,甲醇-水-冰醋酸(8:5.5:1)为流动相,检测波长302nm。结果:水杨酸在1.53-30.54μg·mL-1(r=0.9999)的浓度范围内线性关系良好;方法平均回收率为99.92％,RSD为0.77%(n=6)。结论:本方法快速、准确,可用于检查小剂量阿司匹林肠溶片中的游离水杨酸。

Objective:To establish a HPLC method for testing free salicylic acid in enteric-coated tablets of small dose of aspirin. Methods: The test was conducted on a Diamonsil?C18 column (150mm × 4. 6mm, 5μm) , using methanol-H2O-glacial HAc (8:5. 5:1) as mobile phase and a UV detector at 302nm. Results: The linear range for the method was 1. 53-30. 54μg · mL-1 (r=0.9999). The average recovery was 99. 92 % with a RSD of 0.77% (n= 6). Conclusion: A rapid and accurate HPLC method for testing free salicylic acid in enteric-coated tablets of small dose of aspirin is established .