原发性高血压患者的β-纤维蛋白原-455G/A基因多态性研究

β-Fibrinogen-455G/A Gene Polymorphism in Essential Hypertension

目的 探讨 β-纤维蛋白原 - 4 5 5G/A基因多态性在山东地区汉族人群的分布以及该多态性与原发性高血压病的关系。方法 应用聚合酶链反应和限制性内切酶片段长度多态性技术 (PCR RFLP)检测了 14 8例健康人和 14 9例原发性高血压患者的 β Fg 4 5 5G/A基因多态性 ,比浊法测定血浆纤维蛋白原水平。 结果 β Fg 4 5 5G/A基因多态性分布在患病组和对照组均符合Hardy Weinberg平衡定律。在两组内A等位基因携带者血浆纤维蛋白原水平均显著高于GG基因型者。A等位基因频率在高血压组 (0 171)略高于正常对照组 (0 15 2 ) ,但差别无统计学意义 (P >0 0 5 )。结论 β Fg 4 5 5G/A基因多态可能不是原发性高血压的遗传易感标志。

Objective To explore the β-fibrinogen (Fg) gene polymorphism in Chinese Han population and the association of the polymorphisms with essential hypertension(EH). Methods The β-Fg-455G/A gene polymorphism was identified by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) in 149 patients with EH and 148 healthy controls matched by age and sex. Turbidimetric assays were performed to measure the plasma fibrinogen levels. Results The distribution of β-Fg-455G/A gene polymorphism was in accordance with the Hardy-Weinberg equilibrium( P >0.05). The A-allele is associated with elevated plasma fibrinogen levels in both hypertensives and controls. The A-allelic frequency in EH group and the control group were 0.171 and 0.152. No significant difference was found between them( P >0.05). Conclusion There is no association between the β-Fg-455G/A gene polymorphism and essential hypertension.