血管紧张素转换酶基因和糜酶抑制剂基因多态性与原发性高血压患者左室肥厚的相关性研究

Association Between the I/D Polymorphism of the ACE gene,A/B Polymorphism of the Chymase Gene and Left Ventricular Hypertrophy in Essential Hypertension

目的 探讨血管紧张素转换酶 (ACE)基因插入 /缺失 (I/D)多态性和Chymase(CMA)基因A/B多态性与原发性高血压患者左室肥厚的关系。方法 应用聚合酶链反应 (PCR)和限制性片断长度多态性方法 (RFLP)检测了 10 4 2例原发性高血压患者ACE基因I/D多态性以及CMA基因A/B多态性 ;同时超声心动测量左室舒张末期内径(LVDd)、舒张期室间隔厚度 (IVST)及左室后壁厚度 (LVPWT)。结果 ①ACE基因II、ID、DD基因型及I、D等位基因频率在原发性高血压合并左室肥厚组 (LVH)与无左室肥厚组 (NLVH)间的分布差异无统计学意义 ;②CMA基因AA、AB、BB基因型及A、B等位基因频率在LVH组与NLVH组间的分布差异无统计学意义 ;③ACE和CMA基因型间年龄、体质指数 (BMI)、脉搏、收缩压 (SBP)、舒张压 (DBP)、LVDd、IVST、LVPWT、LVM以及LVMI差异均无统计学意义 ;④ACE和CMA基因型与左室肥厚 (LVH)不相关 ;⑤ACE基因中各基因型与CMA基因中各基因型间不存在交互作用。结论 ACE基因I/D多态性及CMA基因A/B多态性与原发性高血压患者左室肥厚不相关。

Objective To investigate whether the insertion/deletion (I/D) polymorphism of the angiotensin converting enzyme (ACE) gene and the A/B polymorphism of the chymase (CMA) gene was associated with left ventricular hypertrophy (LVH) in Chinese essential hypertension. Methods 1042 patients with essential hypertension were studied. The DNA fragments of ACE gene and CMA gene were amplified by PCR and analysed by RFLP. LVDd,IVST and LVPWT were measured by Ultrasonic Cardiogram (UCG). Results ①The frequencies of the ACE genotypes and I,D alleles were not significantly different between patients with LVH and NLVH. ②The frequencies of the CMA genotypes and A,B alleles were not different between patients with LVH and NLVH. ③No significant differences of age,BMI,pulse,SBP,DBP,LVDd,IVST,LVPWT and LVMI were found in the different genotype groups. ④The genotypes of the ACE and CMA were not related with LVH. ⑤No interaction between the genotypes of the ACE and the genotypes of the CMA. Conclusion No evidences are found to support the association between ACE genotype as well as CMA genotype and the LVH in essential hypertension.