植物系统获得抗性激活剂筛选方法探讨

Studies on Screening Method of Plant SAR

在植物系统获得抗性(SAR)激活剂筛选中,可采用离体测定和活体盆栽测定相结合的方法进行。在离体测定时,不同植物病原细菌和病原真菌,用平板稀释法测定药物对病原物的抑菌活体;活体盆栽测定可在待测试作物水稻、黄瓜、玉米、番茄等作物上叶面喷雾药液,并于喷药当天及3 d、1周、2周后接种植物病原细菌、真菌和病毒,置适宜条件下培养至空白对照明显发病,考察药物对各病害的防治效果。通过离体活性与盆栽效果综合分析,若无离体活性或离体活性较弱,但盆栽药效持久且有广谱效果的药物,即有可能具有SAR激活剂作用。

The combination of in vitro with in vivo test could be adopted in the screening of plant SAR (systemic acquired resistance) activators. In the in vitro test, different phytopathogenic bacteria & fungi were used to test the inhibitted activity of agrochemicals on bacteria & fungi with the method of plate dilution, while in pot test after the spray of the agrochemicals on the leaves of cucumber, corn, tomato and other tested crops, the phytopathologenic bacteria & fungi and virus were inoculated to the host plants on the day of spray and 3days、one week、two weeks after spray, and they were cultured under suitable control to infected obviously. In comprehensive consideration of the result of in vitro and in vivo test, it was suggested that agrochemicals without or with little in vitro activity and with persistence and wide spectrum of pathogens control on pot test were possibly the plant SAR activators.