神经干细胞与自体筋膜联合修复家兔面神经损伤

Research on repairing facial nerve injury of rabbits by neural stem cells and autologous fasia

目的观察应用神经干细胞和自体筋膜修复面神经损伤的效果。方法 2 2只健康新西兰日系大耳白兔 ,随机分为对照组 (单纯自体筋膜移植 )和治疗组 (自体筋膜 +神经干细胞移植 ) ,对照组 8只 ,共 15侧 ;治疗组 14只 ,共 2 0侧。分别于术前、术中(切断前后 )、术后 6周以阈刺激记录其潜伏期。术后 6周取桥接处的再生面神经 ,脱水、石蜡包埋 ,连续切片 (厚度 5 μm ) ,MBP髓鞘染色。半薄和超薄切片 ,分别观察髓鞘的数目和厚度。结果电生理阈刺激下 ,神经潜伏期术前、术中切断前无显著性差异。与术后 6周比较有显著性差异 (P <0 0 5 )。术后 6周取桥接处的再生神经 ,BrdU免疫荧光染色显示 :治疗组有大量BrdU阳性细胞。对照组未见BrdU阳性细胞。MBP免疫组织化学染色显示 :邻片与大量BrdU阳性细胞相对应区有大量MBP阳性细胞 ,横断面为环形或半环形 ,纵断面为连续或不连续的条索状。对照组MBP阳性细胞数目显著减少。半薄和超薄切片显示治疗组的再生纤维以有髓神经纤维为主 ,髓鞘板层结构明暗相间 ,结构清晰 ,轴浆内细胞器丰富。对照组的再生纤维髓鞘发育差 ,但能看到典型的髓鞘板层结构 ,轴浆内细胞器较少。结论神经干细胞和自体筋膜联合应用能显著提高面神经损伤后修复的效果。

ObjectiveTo observe the effect on repairing facial nerve injury of rabbits by neural stem cells and autologous fasia. Methods22 rabbits with transected facial nerve were divided into 2 groups randomly, control group (8 rabbits,15 sides totally), which transected facial nerve were wrapped by autologous fasia, and treament group (14 rabbits, 20 sides totally), which were wrapped by neural stem cells and autologous fasia. Six weeks after transplantation, neuro-electrophysiological test, immunohistochemical examination were done. The number and thickness of myelin in the re-connected area of transected facial nerve were observed. ResultsThe transplanted animals recovered much better than that in control group ( P <0.05). Immunohistochemical examination showed a great deal of BrdU positive cells around the re-connected area of transected facial nerve. Immunohistochemical staining also found plenty of regenerative myelins in this area in the treatment group. While in control group, there were no BrdU positive cells and only a few of regenerative myelins in the same area. ConclusionTransplantation of neural stem cells combined with autologous fasia might become the new method to treat facial nerve injury.