单纯性肥胖儿童外周血单核细胞载脂蛋白E基因的表达

Apolipoprotein E gene expression in peripheral blood monocyte in children with obesity

目的 探讨单纯性肥胖儿童外周血单核细胞载脂蛋白E基因表达及其与血脂、脂蛋白、载脂蛋白的相关关系。方法 采用竞争性逆转录 聚合酶链式反应方法分析 3 2例单纯性肥胖儿童和 3 2例正常健康儿童外周血单核细胞的载脂蛋白E基因表达。结果 载脂蛋白E基因能在儿童外周血单核细胞表达 ,与健康儿童比较 ,单纯性肥胖儿童外周血单核细胞载脂蛋白E基因表达水平显著下调 (P <0 0 1) ,重度肥胖儿童尤其明显 ,载脂蛋白E基因表达水平与肥胖度呈负相关 (P <0 0 5)。肥胖儿童存在明显的血脂代谢紊乱 ,载脂蛋白E基因表达水平与低密度脂蛋白 胆固醇呈负相关 ,与血载脂蛋白E浓度呈正相关 (P <0 0 5) ,与血总胆固醇、甘油三酯、高密度脂蛋白 胆固醇、脂蛋白 (a)、载脂蛋白AⅠ水平无明显相关 (P >0 0 5)。结论 单纯性肥胖儿童外周血单核细胞载脂蛋白E基因表达水平明显下调 ,并与肥胖程度及血脂代谢异常相关联 。

Objective Coronary heart disease (CHD) is one of the most common causes of death in the world Some studies suggested that CHD begins in childhood Obesity and dyslipidemia are important risk factors of coronary heart disease Apolipoprotein(apo)E gene associated with dyslipidemia and coronary heart disease The present study was designed to investigate the expression status of apoE gene in peripheral blood monocyte and association of apoE gene expression with lipids in children with obesity Methods Among 32 children with obesity and 32 healthy children without obesity or overweight, ApoE gene expressions were determined by competitive reverse transcription-polymerase chain reaction in peripheral blood monocyte The concentrations of plasma triglyceride, total cholesterol,low density lipoprotein-cholesterol, high density lipoprotein-cholesterol, lipoprotein(a), apoAⅠ, apoB 100 and apoE were measured Results Expression of apoE gene was detected in peripheral blood monocyte Expression of apoE gene was significantly reduced in children with obesity as compared with control group (0 29±0 14 moles/mole GAPDH mRNA vs 0 36±0 10 moles/mole GAPDH mRNA, t=2 15,P<0 05) The more severe was the degree of obesity, the more significantly reduced the expression of apoE gene; the degree of obesity was negatively correlated with the levels of expression of apoE gene(correlation coefficient =-0 40,P<0 05) Compared with control group, the levels of triglyceride, total cholesterol, low density lipoprotein-cholesterol, and apoB 100 were higher, and those of high density lipoprotein-cholesterol, apoAⅠ and apoE were lower in children with obesity [(1 68±0 50)mmol/L vs (0 99±0 54)mmol/L, (4 47±0 91)mmol/L vs (3 33±0 90)mmol/L, (2 23±0 71)mmol/L vs (1 13±0 96)mmol/L, (94 48±9 97)mg/dl vs (83 81±15 64)mg/dl, (1 47±0 39)mmol/L vs (1 73±0 36)mmol/L, (112 71±27 86) mg/dl vs (134 80±45 36)mg/dl, (24 50±10 92)mg/L vs (35 07±9 79)mg/L, respectively, P<0 05] ApoE gene expression was associated with plasma lipids metabolism in children with obesity The quantity of apoE gene expression was inversely associated with low density lipoprotein-cholesterol,positively correlated with apoE (correlation coefficient =-0 33, 0 35,respectively, P<0 05). The quantity of apoE gene expression was not associated with total cholesterol, triglyceride, high density lipoprotein-cholesterol, lipoprotein(a),apoAⅠ,and apoB 100 (correlation coefficient=-0 19, -0 11, 0 16,0 09,0 18,0 22,P>0 05) Conclusion Expression of apoE gene was significantly reduced in peripheral blood monocyte in children with obesity The quantity of apoE gene expression was associated with degree of obesity and abnormality of blood lipids