肝纤维化大鼠肝表面温度与肝组织形态学变化的相关性研究

Correlation study on the liver surface temperature and liver histological changes in hepatic fibrosis rat induced by CCl_4

目的 :探讨肝纤维化发生、发展过程中肝脏温度的变化规律。方法 :采用红外热像和组织芯片技术 ,研究四氯化碳所致大鼠肝纤维化模型肝脏表面温度与肝组织病理变化的关系。结果 :2w模型组大鼠肝组织病理变化以炎症、肝细胞脂肪变性为主 ,肝表面平均温度较对照组明显升高 (P <0 0 5 ) ;4w模型组在肝细胞变性、坏死的同时 ,ECM增生 ,肝纤维化逐渐形成 ,模型组与对照组肝表面温度无明显差异 ;6w模型组肝细胞大量坏死 ,中央静脉及汇管区纤维化隔形成 ,肝表面温度较对照组明显降低 (P <0 0 5 ) ;8w模型组肝组织形成完整的假小叶 ,肝表面温度较对照组明显降低 (P <0 0 1)。结论 :肝纤维化发生。

Objective:To investigate the changes of liver temperature in hepatic fibrosis rat induced by CCl 4.Methods:The liver surface temperature was detected by computer assisted infrared thermorgraphic technology.The liver histological changes were observed by tissue microarray.Results:In 2w model group,the liver tissue appeared inflammation and mild denaturation and necrosis of hepatocytes.The liver surface temperature increased observably compared with the control group( P <0 05).In 4w model group,there were denaturation and necrosis of hepatocytes and increase of ECM.The liver surface temperature no changes compared with the control group.In 6w model group,there were large numbers of necrosis hepatocytes and a significant increase of ECM around central vein and protal area.The liver surface temperature decreased observably compared with the control group( P <0 05).In 8w model group,there was an obvious nodular appearance and the liver surface temperature decreased observably compared with the control group( P <0 01).Conclusion:The change of liver surface temperature is correlative with liver histological changes in hepatic fibrosis rat induced by CCl 4.