增生性瘢痕成纤维细胞生长特性的研究

The study of the growth behavior of hypertrophic scar fibroblasts versus normal skin fibroblasts

目的 :研究增生性瘢痕成纤维细胞与正常皮肤成纤维细胞生长特性的差异。方法 :应用消化法建立成纤维细胞系 ,在倒置相差显微镜下观察细胞形态 ,应用细胞计数方法绘制细胞生长曲线 ,测定细胞生长速度。结果 :增生性瘢痕成纤维细胞比正常皮肤成纤维细胞胞体大 ,形态不规则。前者细胞倍增时间约为 6d ,后者细胞倍增时间为 3d。细胞融合后前者细胞数为后者的 5 6%。结论

Objective:To study the differences between the growth behavior of hypertrophic scar fibroblasts(HSF) versus normal skin fibroblasts (NSF).Methods:The fibroblasts were established with digesting method.The cell morphology was observed under optic microscope.The cell growth curves were drawn and the growth rates were determined.Results:The hypertrophic scar fibroblasts with more branches protruding from the cell bodies were bigger than the normal skin fibroblasts.The cell double multiplying time of the hypertrophic scar fibroblasts was 6 dyas,much longer than the normal skin fibroblasts.Whereas the ultimate cell number of the former was 56% of the later.Conclusion:The hypertrophic scar fibroblast is a different phenotype from the normal skin fibroblast.