短梗霉高产菌的选育及其10立升罐发酵

10 L Jar Fermentationof Aureubasidium Pullulan N435.3

利用诱变手段获得转化率较高的N435.3号菌株,其摇瓶发酵转化率为45.0％左右。利用10立升罐发酵其转化率为44.3％,利用流加糖手段可将转化率提高至52.2％。

A mutant strain N435. 3 of aureobasidium pullulan 3. 2756 was obtained by mutigenesis. By shaking culture and jar fermentation,the pullulan conversion rates were 45. 0% and 44. 3% respectively. A higher conversion rate 52. 2% was obtained by 10 L jar fed-batch fermentation.