影响中药三七HPLC指纹谱的若干因素

Several Factors Affecting the HPLC-Fingerprinting of Panax notoginseng

目的 :考察三七药材的不同干燥方法、药材干燥程度和保存时间等因素对三七HPLC指纹图谱的影响。方法 :采用三七HPLC指纹谱的方法 [Luna 5 μC18(2 5 0× 4 6mm) ,乙腈 水梯度洗脱 ,流速 1ml/min ,检测波长 2 0 0nm]来分析运用不同干燥方法得到的三七样品以及不同干燥程度和不同保存时间的三七样品 ,揭示这些因素对三七HPLC指纹谱的影响。结果 :①在不同干燥方法下 ,有的组分受到干燥温度和时间的影响较大 ,有的组分受到药材状态的影响较大 ,有些组分同时受到上述两种因素的影响。② 15号色谱峰为新鲜药材和干燥过程中的药材的特征峰 ,并且其变化是可逆的。③在保存过程中 ,10号和 12号色谱峰的峰面积在保存过程中持续上升 ,其余多数色谱峰的峰面积则随保存时间的延长而逐渐降低 ;不同状态的三七药材 ,粉末药材变化幅度较大 ,而整主根药材中组分的变化幅度较小。结论 :干燥方法。

AIM:To study how can the way and the degree of dryness and the period of storage of crude drug samples affect the HPLC fingerprinting of \%Panax notoginseng\% METHOD: The HPLC fingerprinting method:Luna C 18 analytical column (250×4 6 mm,5 μm);acetonitrile water as gradient eluent with flow rate at 1 0 ml/min, detective wavelength at 200nm Notoginseng samples made in different ways of dryness and samples in different degrees of dryness and different moments of storage were analyzed to discover how can they affect the HPLC fingerprinting of notoginseng Two shapes of notoginseng, notoginseng in integrity and pieces, were dried in shade and by baking (35 ℃) respectively Notoginseng in integrity, in pieces and in powder which had been stored for 0 days, 10 days, 20 days, 30 days(1 months), 2 months, 4 months, and 6 months were analyzed individually RESULT: ①Different drying ways work on the components of notoginseng in different ways: some components were affected more by the temperature and period of dryness, which had higher area values of peaks under drying in heat (or in shade);some were affected more by the shape of drug, which had higher peak area value in the shape of pieces (or integrity);some were affected by the both factors mentioned above ②In the experiment, Peak No 15, whose relative peak area is 12 7% 28 1% in fresh and drying drugs, was discovered to be characteristic Its relative peak area was keeping on declining during drying and dropped to 0 2% when the drug was dried completely However the value rose to 0 7%～1 4% when the dried drug was hydrated, so the reaction was concluded to be reversible ③The discipline of changes during storage was found:two peaks(No 10 and No 12) , areas kept on going up during storage, while most others' went down;drug of different shapes changed differently, e g drug in powder changed more rapidly than that in pieces and integrity CONCLUSION:The way and the degree of dryness and the period of storage all can affect the HPLC FPS of notoginseng in a certain extent