食管癌和癌前期病变中GST-π的表达意义

Expression of GST-π in Esophageal Cancer and Precancerous lesion

用抗胎盘型谷胱甘肽S-转移酶(GST-π)抗体的ABC免疫组织化学技术检测了75例食管癌,82例食管上皮异型增生,59例正常食管上皮组织中GST-π活性.食管癌组织中GST-π阳性率为90.6％,食管上皮异型增生组织中73.2％呈GST-π阳性,正常食管上皮组织中3.4％呈GST-π阳性,其余96.6％呈阴性。值得注意是轻度异型增生时,GST-π阳性率为65.4％,而中度和重度异型增生时,其阳性率进一步升高,接近食管癌水平。GST-π在食管癌和癌前病变组织中的异常表达为肿瘤防治研究工作提供了有意义的酶学指标。

Human placental form of glutathione S-transferase (GST-π) was detected in 75 esophageal cancer, 82 dysplasia and 59 normal epithelium of esophagus by avidin biotin peroxidase complex method using anti-GST-π antibody. Of 59 normal epithelium of esophagus, 96.6％ were negative for GST-π staining, 90.6％ of 75 esophageal carcinomas and 73.2％ of dysplasia were positive for GST-π staining. These results indicate that GST-π may be a useful marker for immunohistochemical detection of human esophageal cancer and precancerous lesion.