心肌缺血再灌注性收缩带的形成与细胞膜损伤

Formation of contraction band and injury role to sarcoplasma after reperfusion following myocardial ischemia

用离体灌注的大鼠心模拟局部缺血及再灌注探讨再灌注对肌纤维的损伤和促进收缩带形成的作用.表明收缩带起源于交界性损伤阶段的肌纤维.将收缩带分为节段、条带、枝条和碎片四型,延长缺血及再灌注时间,则前两型数量减少而第三型数量增加,肌纤维挛缩时肌节极度缩短,导致横管牵拉胞膜,易造成膜破裂.加速肌纤维坏死.

Reperfusion following myocardial ischemia was imitated using perfused isolated rat heart, to explore the injury role of reperfusion to myofibril and the course of contraction band (CB)formation, and investigate the origin of CB as well as division of its type. It was found that CB originated from injury cells which located border line stage; CB can be divided into section type, strip type, twig type and piece type; as ischemia or reperfusion time elongating percent of section type and strip type gradually decreased but twig type increasingly rose; during the process of CB formation, sarcomere was shorted violently, cytomembrane was pulled by transverse tuber, and sarcoplasma was easily teared, cellular necrosis was accelerated.