AT_1R基因1166位点多态性与原发性高血压患者生化指标关系的研究

The study of the relation between the AT_1 receptor gene polymorphism and some biochemical indices of essential hypertension patients

目的 :探讨血管紧张素Ⅱ 1型受体 (AT1R)基因A116 6 /C多态性与国人原发性高血压 (EH)患者部分生化指标之间的关系。 方法 :6 4例轻中度EH患者住院期间停用所有降压药物 5个半衰期后 ,测量患者基础血压 ,并抽取血标本 ,应用限制性内切酶酶解聚合酶链反应 (PCR RFLP)的方法检测AT1R基因型 ,同时进行身高、体重、空腹血糖 (Glu)、血清总胆固醇 (TC)、三酰甘油 (TG)、高密度脂蛋白 (HDL C)、低密度脂蛋白 (LDL C)、尿素氮(BUN)、肌酐 (Cr)、尿酸 (UA)浓度测定。 结果 :CC基因型因其突变率极低 ,本次试验未发现 ,故未予分组。①本组EH患者AC基因型频率为 2 3.4 % ,C等位基因频率为 11.7% ;②TG浓度AC型较AA型低 ;③AC型SCr、UA浓度较AA型者高 ;④空腹Glu、TC、HDL C、LDL C、BUN浓度两基因型间无显著差异。 结论 :在血脂水平上 ,A116 6 /C多态性可能与EH患者TG水平相关 ;A116 6 /C多态性可能与患者的肾功能相关 ,AC型患者肾功能可能较AA型差 ;A116 6 /C多态性可能与EH患者的空腹Glu、TC、HDL C、LDL C。

Objective:To investigate the relation between the AT 1 receptor gene polymorphism and some biochemical indices of essential hypertension patients. Methods:Sixty-four patients of mild-moderate essential hypertension were not given any anti-hypertension drugs for 5 half lives,then used the PCR-RFLP to detect the AT 1 receptor gene type and measured the basic blood pressure at the same time. After that, fasting serum glucose , serum total cholesterol(TC) , triglyceride(TG) , high-density lipoprotein cholesterol(HDL-C) , low-density lipoprotein cholesterol(LDL-C) , blood urea nitrogen(BUN) , creatinine(Cr) were measured in all subjects. Results:①The frequency of AC gene type in these essential hypertension patients and the C1166 allete of AT 1 receptor gene was 23.4% and 11.7%. ②There was a remarkable difference on TG between two gene groups. ③There were obvious difference on Cr and UA between two gene groups .④There were no obvious difference on Glu, TC ,HDL-C ,LDL-C ,BUN between AC and AA gene type. Conclusion:①The concentration of TG of AC gene type is lower than that of AA , there may be relativity between two gene type. ② A1166/C gene polymorphism may be associated with renal function. AA gene type may have poorer renal function. ③A1166/C gene polymorphism may be not relative with Glu,TCH ,HDL-C ,LDL-C ,BUN.