对不同的卵巢上皮性腺癌细胞株建立裸鼠动物模型的实验研究

Experimental study on establishing tumor bearing nude mice model with different ovarian adenocarcinoma cell lines

目的 :探讨卵巢上皮性腺癌细胞株建立裸鼠动物模型的最佳方案。 方法 :采用两种卵巢上皮性腺癌细胞株CAOV3(贴壁细胞 )、COC2 (悬浮细胞 )对裸鼠进行不同细胞密度皮下接种及不同方法的组织块接种。 结果 :在 5× 10 6/ (0 .2 0ml·只 )到 6× 10 7/ (0 .2 0ml·只 )细胞浓度内 ,两株细胞的成瘤率及肿瘤成形时间与接种细胞量无关。与COC2细胞株相比 ,CAOV3成瘤早 (以组织块接种者为甚 ) ,成瘤率高 ,成瘤后生长缓慢。而COC2细胞株一旦成瘤 ,则肿瘤生长速度极快 ,以至于实验尚未完成荷瘤鼠已死亡。 结论 :在建立卵巢上皮性腺癌裸鼠动物模型的实验中 ,以选用贴壁细胞株。

Objective:To establish the best way of tumor-bearing nude mice model with epithelial ovarian adenocarcinoma cell lines. Methods:CAOV3 (wall-attached cell) and COC2 (suspended cell) cell lines were transplanted subcutaneously to the nude mice by injection with different cell concentration or tissue block implantation. Results:The rate and time of tumor formation in the two cell lines were not related to cell concentration in the range from 5×10 6/0.2 ml to 6×10 7/0.2 ml. Time of tumor formation was earlier(especially in the manner of tissue block implantation),the successful rate of tumor formation was higher and the speed of tumor growth was relatively retarded in the nude mice bore CAOV3 cell line's tumor than that in COC2 cell line's. But once tumors were formed in nude mice bearing COC2 cell line, the mice died too fast to make experiment because of very quick speed of tumor growth. Conclusion:Wall-attached cell line, tissue block implantation with trocar may be the best way to establish tumor-bearing nude mice model in epithelial ovarian adenocarcinoma cell lines.