早产儿血浆中还原型谷胱甘肽含量的测定

Detection of the plasma concentration for glutathione (GSH) in premature infants

目的 通过测定早产儿血浆中还原型谷胱甘肽 (GSH)含量 ,评价早产儿体内的抗氧化能力。方法 将 2 0例临床及X线表现排除RDS的早产儿及 10例正常足月儿 ,分别于 0d、3d采静脉血 2ml ,测定两组新生儿外周血浆中GSH的含量 ,比较两组婴儿之间及早产儿 0d、3d时GSH含量。结果 早产儿出生时GSH含量明显低于足月儿 (P <0 .0 1) ,且早产儿 3d时GSH含量较 0d时明显降低 ,差异显著 (P <0 .0 5 )。结论 早产出生总是伴有GSH水平低下 ,且生后 2 - 3d达到最低值 ,这将影响其肺脏的抗氧化防御能力。因此 ,早产儿生后需及时补充GSH。

Objective: To assess the plasma concentration of GSH in premature infants. Methods: Twenty premature infants entered the study, who did not have clinical or radiological signs of RDS and not or required oxygen supplemention<48h, maximal FiO 2<0.4. The control group consisted of 10 normal term infants. heparinized blood was sampled on 0d,3d,repecively, and the blood was centrifuged (750g,10min) within 15min after collection and the plasma stored at -20°C. Results: At birth, the plasma concentration of premature infants were significantly lower than that of term infants(P<0.01). In premature infants, the level of GSH on 3d was lower than that of GSH on 0d(P<0.05), and plasma GSH concentration reached a nadir on day 2 or 3. Conclusion: GSH deficiency is present in prematures and that it increases with the degree of prematurity. At birth, GSH deficiency will compromise the lungs defense against oxidative stress injury.