武汉、泉州两地肺炎克雷伯菌产β-内酰胺酶类型的比较研究(英文)

Study on classification and distribution of β-lactamase produced by Klebsiella pneumonia in Wuhan and Quanzhou

目的 比较研究武汉、泉州两地肺炎克雷伯菌 (Kpn)所产各种β -内酰胺酶 (β -lase)分布情况。 方法 采用改良多底物相邻纸片协同法检测武汉、泉州各 70株Kpn所产各种 β -lase。 结果 武汉 70株Kpn总 β-lase检出率为 2 8.7% ,其中产青霉素酶、头孢菌素酶各 1株 (1.4 % ) ,产广谱酶 4株 (5 .7% ) ,产ESBLs14株 (2 0 .0 % ) ,未检出培南酶 ;14株产ESBLs菌株中头孢他啶、氨曲南、头孢噻肟为底物的分别为 11株、11株、12株。泉州 70株Kpn总 β -lase检出率为 85 .7% ,其中产青霉素酶 4株 (5 .7% ) ,头孢菌素酶 3株 (4.3% ) ,产广谱酶 11株 (5 .7% ) ,产ESBLs4 2株 (6 0 .0 % ) ,未检出培南酶 ;4 2株产ESBLs菌株中头孢他啶、头孢噻肟、氨曲南为底物的分别为 34、38、4 0株。结论 本组武汉、泉州Kpn所产各种 β -lase均以广谱酶、ESBLs为主 ,产ESBLs菌株的头孢他啶、头孢噻肟、氨曲南检出率均不相同 ,武汉以头孢噻肟最高 ,泉州以氨曲南最高 ;均未检出培南酶 ;武汉、泉州两地Kpn所产各种 β -lase的产酶率、类型不同 ,泉州Kpn总产酶率、各种 β -lases均高于武汉Kpn。

Objective:To study the Classification and Distribution of the β-Lactamases Produced by Klebsiella pneumonia in Wuhan and Quanzhou.Methods:To detect the β-Lactamases produced by Klebsiella pneumonia in Wuhan and Quanzhou with a multi-disk synergy test.Results:20 of 70 strains produced the β-Lactamases in Wuhan,total producing rate was 28.7%,each strains produced penicillinase or cephalosporinase,4 strains produced broad-spectrum β-Lactamase(BSBLs,5.7%);14 strains produce extented-spectrum β-Lactamase(ESBLs,20.0%), the sensitivities of the ceftazidime,aztreonam and cefotaxime were different,cefotaxime was the highest. 60 of 70 strains in Quanzhou produced the β-Lactamases,total producing rate was 85.7%,4 strains produced penicillinase,3 strains produced cephalosporinase;11 strains produced BSBLs(15.7%),42 strains produced ESBLs(60.00%),the sensitivities of the ceftazidime,aztreonam and cefotaxime were different,aztreonam was the highest. All did not produce carbapenem-hydrolyzing β-Lactamase(CHBLs).Conclusions: The types of β-Lactamase produced by Klebsiella pneumonia in Wuhan and Quanzhou are mainly BSBLS and ESBLS, the sensitivities of the ceftazidime,aztreonam and cefotaxime were different,in wuhan Cefotaxime was the highest,in Quanzhou aztreonam was the highest; all did not produce carbapenem-hydrolyzing β-Lactamases (CHBLs).The rate and types of β-Lactamases Produced by Klebsiella pneumoniae in Wuhan and Quanzhou were different, the total producing rate and the producing rate of each type of β-Lactamases produced by Klebsiella pneumoniae isolates from Quanzhou were higher than that from Wuhan.