乙型肝炎患者血清TNF -α、IL-6、IL-8、IL -12、IL-18变化的研究(英文)

Change of serum TNF-α, IL-6, IL-8, IL-12 and IL-18 in patients with hepatitis B

目的 探讨血清细胞因子水平与乙型肝炎病变的关系。方法 运用ELISA法、比色法及荧光定量PCR技术检测不同类型乙型肝炎患者血清细胞因子、ALT、TBil及IBV -DNA水平 ,观察患者治疗前后细胞因子变化及其与预后的关系。结果 除隐匿性肝炎外 ,急性、慢性及重型肝炎患者血清TNF -α、IL - 6、IL- 8、IL - 12、IL - 18的水平均显著高于对照组 (P <0 .0 0 1) ,升高程度与病情相关 ;急性、慢性肝炎经治疗后 ,细胞因子浓度均明显下降 (P <0 .0 0 5～ 0 .0 0 1) ,重型肝炎患者无明显变化 (P <0 .0 5 ) ;预后好的患者细胞因子水平下降更为明显 (P <0 .0 5 ) ;相关分析表明 :乙肝患者各细胞因子与血清ALT、TBil水平相关 ,与HBV -DNA无相关性。结论 血中细胞因子水平是判断乙肝患者免疫状态、肝脏损伤及评估预后的重要指标。

Objective:To investigate the relationship between serum cytokines and hepatitis B.Methods:The levels of serum cytokines, ALT, TBil and HBV-DNA were measured in 96 patients with hepatitis B and 22 normal controls by ELISA, colorimetry and quantitative fluorescence PCR technique.Results:Except latent hepatitis, the levels of serum TNF-α,IL-6,IL-8,IL-12 and IL-18 in different types of hepatitis B were significantly higher than that of controls (P<0.001). The cytokines in patients with acute and chronic hepatitis B were significantly decreased after treatment(P<0.005, P<0.001)and the patients with favourable prognosis had significantly lower levels than those patients with unfavourable prognosis(P<0.05).Serum levels of cytokines were positively correlated with the ALT, TBil (r:0.51～0.73, P<0.05) and weren't correlated with HBV-DNA (r:-0.028～0.049, P>0.05).Conclusions:Blood cytokines could reflect the state of immunity,liver injury and prognosis.