脑溢安对试验性脑出血大鼠血管内皮生长因子表达的影响

Effect of Nao Yi An on VEGF Expression in Rat Brain with Cerebral Hemorrhage

目的 研究实验性脑出血大鼠脑内血管内皮生长因子 (VEGF)的时空分布及中药脑溢安的影响。方法 通过立体定位向脑内苍白球注入Ⅶ型胶原酶 0 .4U建立脑出血模型 ,运用免疫组化和原位杂交法检测脑出血后 3h、6h、12h、1d、4d、7d ,14d七个时间点VEGF的表达变化 ,以阳性细胞数作为观察指标。结果 脑出血 3hVEGF开始表达 ,6h增强 ,12h及 1d阳性细胞达到高峰 ,4d开始下降 ,到 7d和 14d仍有少量表达。脑溢安组在 6h、12h、1d、4d高于模型组 (P <0 0 5 )。结论 脑出血后脑内VEGF表达增加 ;脑溢安可上调脑出血后VEGF的表达 ,可能是其神经保护机制之一。

Objective To study expression change of vascular endothelial growth factor(VEGF) protein and mRNA in rat brain with cerebral hemorrhage and effect of traditional Chinese medicine complex Nao Yi An(NYA) on VEGF expression. Methods The intracerebral hemorrhage(ICH) animal models were made by injecting collagenase type Ⅶstereotaxically into right globus pallidus. The experiment techniques of immunohistochemistry and in situ hybridization were employed to detect the expression variation of VEGF at 3h,6h,12h,1d,4d,7d and 14d after cerebral hemorrhage by positive cell counting as an observation index. Results The VEGF expression in the rat brains was observed at 3h after cerebral hemorrhage, raising at 6h,peaking at 12h and remaining peak until 1d. In the fourth day, VEGF began to decrease and still expressed a little at 7d and 14d.NYA could increase the VEGF expression in rat brains with cerebral hemorhage obviously at 6h ,12h,1d and 4d (P<0 05). Conclusions VEGF expression increased following ICH and NYA could up-regulate the VEGF expression, which may be one of mechanisms of NYA neuroprotective role.