大肠腺癌中Survivin表达及与VEGF的相关性研究

Expression of Survivin Gene and Its Correlation with VEGF Expression in Colorectal Adenocarcinoma

目的 探讨survivin在大肠腺癌发病机制中的作用及其与VEGF在肿瘤转移中的关系。方法 对 42例大肠腺癌组织用逆转录PCR方法检测survivinmRNA表达 ,免疫组织化学SP法检测VEGF蛋白表达。结果 大肠腺癌组织survivin基因表达阳性率为 5 4 8%,癌旁正常组织均无表达。survivin表达与大肠腺癌分化程度、Dukes分期明显相关 ,而与性别、淋巴结转移无相关关系。VEGF表达与大肠腺癌淋巴结转移显著相关。survivin与VEGF的表达具有一致性 ,两者同时表达的大肠腺癌更容易发生淋巴结转移。结论 survivin基因表达与大肠腺癌临床病理特征和生物学行为有密切关系 ,检测survivin的表达水平 ,对于大肠腺癌的诊断。

Objective To investigate the role of Survivin gene in the pathogenesis of colorectal adenocarcinoma and its correlation with VEGF in tumor metastasis. Methods The expressions of Survivin mRNA and VEGF protein were detected by RT-PCR and immunohistochemical staining, respectively, in 42 colorectal adenocarcinoma samples. Results Survivin mRNA expression was positive in 23 cancer samples(54 8%), while no expression was detected in adjacent normal tissue. The expression of Survivin gene significantly correlated with histological differentiation and Dukes stage, but not with gender or lymph node metastasis. The expression of VEGF significantly correlated with lymph node metastasis. The expressions of Survivin and VEGF were coincident. The colorectal adenocarcinomas which expressed both the Survivin and VEGF were more inclined to occur lymph node metastasis. Conclusions Survivin gene expression significantly correlated with the clinico-pathologic characteristics and biologic behavior in colorectal adenocarcinoma. Detection of Survivin expression may be valuable for diagnosing colorectal adenocarcinoma, and evaluating malignancy extent and prognosis.