d-儿茶素-3-O-β-D-葡萄糖苷的抗氧化作用(英文)

Antioxidant effect of d-catechin-3-O-β-D-glycoside

目的 研究粘萎陵菜含有的一种化合物 ,d 儿茶素 3 O β D 葡萄糖苷 (CGS)的保肝作用机制。方法 测定CGS在体外对NADPH 维生素C和Fe2 + 半胱氨酸系统诱发的微粒体脂质过氧化反应(丙二醛形成 )的影响 ,对黄嘌呤 黄嘌呤氧化酶系统超氧阴离子自由基产生 (还原型细胞色素C形成 )的影响 ;在体内对CCl4和乙醇诱发的小鼠肝丙二醛形成的影响。结果 体外CGS 1 0 0 μmol·L-1 能明显抑制NADPH 维生素C和Fe2 + 半胱氨酸系统诱发的大鼠脑、肝、肾微粒体丙二醛形成及黄嘌呤 黄嘌呤氧化酶系统超氧阴离子自由基的产生。在体内能抑制CCl4和乙醇诱发的小鼠肝脂质丙二醛形成。

AIM To investigate the mechanism of d catechin 3 O β D glycoside(CGS), one of the compounds from the root of Potentilla viscosa, on liver protection. METHODS The effects of CGS on the lipid peroxidation〔malondialdehyde(MDA) formation〕 in microsomes of rat brain, liver and kidney induced by NADPH vitamin C and ferrous cysteine systems in vitro, the superoxide anion radical production(reduced cytochrome C formation) in xanthine xanthine oxidase system in vitro, and CCl 4 and ethanol induced MDA formation in mouse liver in vivo were evaluated. RESULTS CGS 100 μmol·L -1 inhibited MDA formation and the superoxide anion radical production in microsomes of rat brain, liver and kidney in vitro , and MDA formation in mouse liver in vivo. CONCLUSION CGS exhibits an antioxidant activity.