三种细菌制剂对草坪草的抗旱和抗病效应研究

Study on Three Species of Bacteria Fertilizer Improving Drought Resistance and Disease Resistance of Turfgrass

本研究以草坪草高羊茅为材料,将实验室分离的三种细菌(E、F、H)制成菌剂,并将三种细菌制剂应用于草坪草,测定其对草坪草抗旱性及抗病性的影响。结果显示,三种菌剂分别与复合肥混合使用效果最佳,可有效促进草坪草生长,减少电解质透出率和丙二醛含量,增加草坪草游离脯氨酸含量、硝酸还原酶含量和叶绿素含量,增强了草坪草的抗旱性。接种立枯丝核菌后,菌剂与复合肥混合使用可推迟草坪草的发病时间并减少病斑数量,过氧化物酶活性、多酚氧化酶活性及苯丙氨酸解氨酶活性均高于其它处理,草坪草对立枯丝核菌的抗性明显增强。

Using Festuca arundinaces as the material, the authors applied bacteria fertilizer E, F and H on the turfgrass, then inspected the effect of bacterial fertilizer on the drought resistance and disease resistance of turfgrass. The result indicated that the turfgrass applied with both bacterial fertilizer and chemical fertilizer grew better than that of other treatments, the contents of proline, nitrate reductase(NR), chlorophyll increased, while electrolyte leakage and the content of MDA declined, the drought resistance of the turfgrass was advanced. After the turfgrass were inoculated by Rhizoctonia solani , the number of disease spot of turfgrass which added bacteria fertilizer and chemical fertilizer were less than that of other treatments, and the contents of peroxidase, polyphenoloxidase and phenylalanine ammonia-lyase were higher than that of other treatment. The resistance of turfgrass on the Rhizoctonia solani was advanced.