三组中(藏)药合剂对大鼠脑组织缺氧的实验研究报告(一)

Research on Protective Effects of three Kinds of Chinese (Tibet) Traditional Medicine Mixture Against Hypoxic Brain Injury in Rats (Ⅰ)

目的 :研究和比较三组中 (藏 )药合剂对大鼠缺氧时神经保护作用。方法 :采用低压舱减压造成SD大鼠缺氧并分成常氧组、低氧组。三组中 (藏 )药合剂 (分别为 1号药组 ,2号药组 ,3号药组 )。检测大鼠低氧暴露后脑组织中谷光甘肽 (GSH)含量及谷光甘肽过氧化物酶 (GSH -PX)和过氧化氢酶 (CAT)的活性。结果 :低氧组与常氧组比较 ,低氧暴露后动物脑组织中GSH含量下降 ,GSH -PX和CAT活性降低 ,使用三种中药合剂后 ,GSH、GSH -PX、CAT均有不同程度的提高 ,除 2号药组CAT升高不明显外 ,其余改变均有显著性意义 (P <0 .0 5或P <0 .0 1)。三个药物之间比较 ,GSH含量相互间无显著性差异 (P >0 .0 5 ) ;对GSH -PX、CAT活性 ,3号药作用最佳 ,1号药次之 ,2号药作用最弱。结论 :三组中 (藏 )药合剂对大鼠缺氧脑组织均有一定的抗氧化神经保护作用。以 3号药最佳 ,1号药次之 。

Objective:To study the anti-oxidative effects of three kinds of Chinese (Tibet) traditional medicine mixture against hypoxic brain injury in rats. Methods:Adult Sprague-Dawley rats were divideded into normoxic group, hypoxia group and three kinds of Chinese traditional medicine mixture pretreatment groups. The hypoxic model established in hypobaric chamber was used to observe the effects of three mixtures on content of GSH, activities of GSH-PX and CAT in brain tissue. Results:Compared with normoxic group, content of GSH, activities of GSH-PX and CAT decreased significantly in hypoxic group, but they were elevated with above three mixtures. Among these three mixtures, mixture number 3 was the best in elevating content of GSH and activities of GSH-PX and CAT.Conclusions:Three kinds of Chinese (Tibet) traditional medicine mixtures play an anti-oxidative neuro-protective role in hypoxia-induced brain damage, mixture number 3 is the best among them.