摘要
选用了甲型流感病毒 10个毒株 (全序列 )和一个 2 0 0 4年越南毒株 (部分序列 ) ,采用自己编写的计算机程序sRNAFinder,对每个毒株的 6个RNA片段的 6个编码序列进行了siRNA设计 .结合系统发育重建方法 ,利用sRNAFind er的共有序列分析功能 ,对得到的 6 10 1个siRNA分子进行了计算机筛选 .结果表明 ,设计的siRNA的分子 ,针对np和pb1应该是最有效的 ,而针对ha和na的siRNA分子设计 ,需要疾病流行期毒株的最新测序数据 ;从ns1的数据不能获得很保守的、可作为预防或治疗药物的siRNA分子 .图 1表 4参
Six complete coding sequences (cds), pb2, pb1, ha, np, na, and ns1, of 10 species of influenza A virus (IAV), including 7 species of H5N1 subtype, 1 species of H1N1 subtype, 1 species of H6N1 subtype and 1 species of H9N2 subtype, and 6 partial cds of a Vietnam species (H5N1 subtype) were scanned using the program sRNAFinder. The 6101 siRNAs were screened using the consensus analysis of the program after phylogenetic analysis (6 phylogeny trees) of the 11 species. The results indicate (i) siRNAs specific for pb1 and np could be appropriate vaccine candidate, (ii) siRNAs specific for ha or na need the sequences of IAV strain isolated from pandemic, (iii) siRNAs specific for ns1 couldn′t be candidate of prophylaxis and therapy for IAV infection. Fig 1, Tab 4, Ref 16
出处
《应用与环境生物学报》
CAS
CSCD
2004年第1期133-138,共6页
Chinese Journal of Applied and Environmental Biology
关键词
甲型流感病毒
SIRNA
系统发育重建
计算机筛选
禽流感
influenza A virus
H5N1 subtype
siRNA
sRNAFinder program
phylogenetic reconstruction
computer screening
