甘西鼠尾草注射液与丹参注射液对化学性缺氧小鼠能量代谢影响的比较

Comparison of energy metabolism on brain lacking oxygen between Salvia miltiorrhiza Bunge injection and Salvia przewalskii Maxim injection in mice

目的 :比较甘西鼠尾草注射液与丹参注射液对急性化学性缺氧小鼠能量代谢的影响。方法 :通过注射NaNO2 造成小鼠急性化学性缺氧 ,记录小鼠存活时间 ,用ATPase、LD检测试剂盒分别检测脑组织中Na+ K+ ATPase、Ca2 + ATPase和乳酸 (LD)含量 ,比较两药对能量代谢的影响。结果 :两药均可延长化学性缺氧小鼠存活时间 ,增强Na+ K+ ATPase。Ca2 + ATPase活力 ,降低LD含量 ,其中大剂量甘西鼠尾草注射液较大剂量丹参注射液更能增强Na+ K+ ATPase活力 ,而小剂量的甘西鼠尾草注射液在增强Ca2 + ATPase活力上较小剂量的丹参注射液作用更强 ;在降低缺氧小鼠脑组织LD含量方面 ,两药作用基本相似。结论 :丹参注射液和甘西鼠尾草注射液对缺氧小鼠的能量代谢均有改善作用。两药均可延长缺氧小鼠存活时间 ,降低脑组织LD含量 ,提高ATPase活力 。

AIM: To compare the influence of salvia miltiorrhiza Bunge njection (SMBI) and salvia przewalskii Maxim injec tion (SPMI) on energy metabolism of acute brain lacking oxygen. METHODS : The whole brain lacking oxygen model was made by injecting NaNO 2 in mice and then wrote down the existent time of mice and detected the contents of Na +-K +-ATPase, Ca 2+ -ATPase and lactic acid (LD) in the brain. RESULTS: SMBI and SPMI both significantly lengthened the existent tim e of mice with the whole brain lacking oxygen, decreased the contents of LD and increased the activity of ATPase in mice. In increasing the activity of ATPase, the influence of SPMI was more than that of SMBI (P< 0.05 ), b ut there was no significant difference between two injections in decreasing the contents of LD (P> 0.05 ). CONCLUSION: SMBI an d SPMI have distinctive protection on energy metabolism of acute brain lacking o xygen. Both of them can lengthen the existent of mice, reduce the contents of LD in brain and increase the activity of ATPase, but SPMI is more excellent than S MBI in increasing the activity of ATPase.