HPCE法测定生黄芩、酒黄芩中黄芩苷含量

Determination of baicalin in Radix Scutellariae and stir-bake with wine Radix Scutellariae by HPCE

目的 :建立中药饮片生黄芩、酒黄芩中黄芩苷含量测定的毛细管电泳法。方法 :毛细管电泳法紫外检测生黄芩和酒黄芩中黄芩苷的含量 ,以 4 0mmol/L硼砂缓冲液 (pH 8.5 )为电泳介质 ,未涂层融硅毛细管 (5 0 μm× 4 7cm) ,有效分离长度 4 0cm ,压力进样 17kPa·s ,2 5kV恒压电泳 ,检测波长为 2 80nm。结果 :黄芩苷的线性范围在 0 .0 5～ 0 .80mg/ml,回归方程Y =2 .1335X - 0 .0 5 77,r=0 .9991,平均回收率 98.96 % ,n =5 ,RSD =1.0 2 %。结论 :本方法快速、简便、结果准确 ,重现性好 ,可用于生黄芩、酒黄芩中黄芩苷的含量测定。

Objective:To develop a method for the determination of baicalin in Radix Scutellariae and stir-bake with wine Radix Scutellariae by HPCE. Methods: Separation was carried out in an uncoated fused silica capillary 50 μm×47 cm ,effective length 40cm. Meanwhile a running voltage 25 kV, 40 mmol/L borax (pH 8.5) buffer and a UV detector at 280 nm adopted. Results: The calibration cruve showed good linearity over the range of 0.05～0.80mg/ml.Regression equation was Y=2.133 5X-0.057 7 ,r=0.999 1.The average recovery rate was 98.96%,n=5,RSD=1.02%. Conclusion: The method is rapid, simple and accurate with good repeatability and it can be used to determine baicalin.