急性髓性白血病肺耐药相关蛋白(LRP)的表达和临床意义

The clinical significance of lung resistance-related protein gene expression in patient with acute myeloid leukemia

目的 :探讨肺耐药相关蛋白 (LRP)基因在急性髓性白血病细胞中的表达及其在临床耐药、预后判断方面的意义。方法 :应用半定量RT PCR技术 ,检测了 6 2例AML患者和 10例正常对照者LRP基因的表达 ,并随访了 10例患者治疗前后LRP基因的表达情况。结果 :M3的LRP基因表达水平和阳性率明显低于M4 、M5(P <0 .0 5 )。难治复发组LRP基因表达水平与正常对照组相比较明显增高 (P <0 .0 1)。初治组中LRP- 患者的完全缓解率明显高于LRP+患者(P <0 .0 5 )。 10例随访患者中LRP阴性者治疗效果和生存期均优于LRP阳性者。结论

Objective:To investigate the relationship between the expression of lung resistance related protein(LRP)gene and multidrug resistance(MDR)in patients with acute myeloid leukemia(AML).Methods:The expression of lung resistance related protein gene was detected in 62 patients with AML and 10 normal subjects by semi quantitative reverse transcriptase polymerase chain reaction(RT PCR),using β actin as internal reference.Results:The expression lever of LRPgene was lower in FAB subtype M 3 than in M 4 and M 5( P <0.05).The expression of LRP mRNA and positive percentage of LRP in relapsed/refractory group were significantly higher than in control group( P <0.01).The complete remission(CR)rate was 82.3 % for patients without LRP expression,but only 42.9 % for patients with LRP expression( P <0.05).LRP gene expression was follow up detected in 10 patients.Survival was longer in patients with LRP - than in patients with LRP +.Conclusion:Overexpression of LRP gene appears to be another clinically relevant drug resistance gene and predicts for poor outcome in AML.