血管新生在多发性骨髓瘤中的临床意义

The Clinical Significance of Angiogenesis in Multiple Myeloma

目的 研究多发性骨髓瘤患者骨髓中血管新生的情况及临床意义。方法 应用免疫组化及原位杂交染色技术 ,对 4 2例多发性骨髓瘤患者治疗前后及 10例正常对照骨髓中微血管密度 (MVD)及血管内皮细胞生长因子(VEGF)表达进行检测。结果 多发性骨髓瘤患者治疗前骨髓中VEGF的阳性率为 5 2 .4 % (2 2 / 4 2 ) ,VEGF阳性组MVD显著高于阴性组 (P <0 .0 1) ;多发性骨髓瘤患者骨髓中MVD及VEGF表达均显著高于正常对照组 (P <0 .0 1) ;多发性骨髓瘤Ⅲ期患者骨髓中MVD显著高于Ⅰ、Ⅱ期患者 (P <0 .0 5 )。多发性骨髓瘤患者治疗前后MVD无显著变化 (P >0 .0 5 )。结论 血管新生及其正性调节因子VEGF在多发性骨髓瘤发生、发展中起着重要的作用。

Objective To investigate the clinical significance of angiogenesis in multiple myeloma.Methods The microvascular density(MVD)and expression of vascular endothelial growth factor(VEGF)in bone marrow biopsies was examined by situ hybridization and immunohistochemical staining in 42 multiple myeloma patients before and after chemothrepy compared with 10 control cases.Results Before treatment,the positive expression rate of VEGF was 52.4%(22/42)and Increased expression of VEGF was significantly correlated with an increase in MVD(P<0.001). MVD and expression of VEGF in multiple myeloma were higher than those in control(P<0.001).MVD in stage Ⅲ was higher than that in stage Ⅰand Ⅱ(P<0.05).MVD did,nt significantly changed after treatmentgroup(P>0.05).Conclusion These findings indicate that the angiogenesis and VEGF migt play an important role in multiple myeloma.