无细胞猪真皮的生物相容性评价

Biocompatibility of Acellular Porcine Dermis

采用猪皮制备的无细胞真皮基质,在基质上接种血管内皮细胞(VEC),鼠成纤维细胞(3T3),在细胞水平检测其生物相容性。采用MTT法检测VEC、3T3在基质上增殖活力;将复合培养1周的VEC-真皮复合物作组织切片,光镜下观察细胞生长状况。结果发现VEC在无细胞猪真皮基质上增殖迅速,并已经形成单层细胞膜片,某些局部区域已形成2～3层细胞膜片。结果说明采用的无细胞猪真皮基质具有良好的生物相容性,该材料可能有广泛的应用前景。

In vitro cultured vascular endothelial cells (VEC) and mouse 3T3 fibroblasts (3T3) on the acellular dermal matrix , which were made of porcine skins. We made the cell proliferation test with MTT assay and the histological observation after cells were seeded on the acelluar dermis for 1 week with histological section. The cell growth curves showed VEC and 3T3 grew much rapidly on the acelluar dermis.The histological observation revealed VEC had formed a monolayer, some places even had formed 2 to 3 layer. The results suggest that the acelluar porcine dermal matrix have good biocompatibility , it will be widely applied.