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Cleavage of hairpin ribozyme to target a transcribed VEGF RNA in vitro

Cleavage of hairpin ribozyme to target a transcribed VEGF RNA in vitro
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摘要 Objective:To constructan expressionvectorbearinghairpinribozyme(Rz)geneagainstvascularendo-thelialgrowthfactor(VEGF)andto assaythecleavageactivityof theribozymein vitro.Methods :Anti-VEGFhair-pinRz genewassynthesizedwhileVEGFgenewasclonedfromhumanplacenta,andtheywerebothinsertedinto theeukaryoticexpressionvectorpcDNA3.ThenpcDNA3-RzandthepcDNA-VEGFweretranscriptedin vitro and cleavageactivityof theRz wasmeasured.Results:Thefragmentof theRz genewasconfirmedby thedigestionof pcDNA3-RzwithEcoR I andBam HI.Theexpressionproductof theRzgenehashighercleavageactivity.Conclusion:WehavesuccessfullyconstructedpcDNA3-Rzexpressionvectorandlaida basisforfurtherstudyof gene therapy. Objective:To constructan expressionvectorbearinghairpinribozyme(Rz)geneagainstvascularendo-thelialgrowthfactor(VEGF)andto assaythecleavageactivityof theribozymein vitro.Methods :Anti-VEGFhair-pinRz genewassynthesizedwhileVEGFgenewasclonedfromhumanplacenta,andtheywerebothinsertedinto theeukaryoticexpressionvectorpcDNA3.ThenpcDNA3-RzandthepcDNA-VEGFweretranscriptedin vitro and cleavageactivityof theRz wasmeasured.Results:Thefragmentof theRz genewasconfirmedby thedigestionof pcDNA3-RzwithEcoR I andBam HI.Theexpressionproductof theRzgenehashighercleavageactivity.Conclusion:WehavesuccessfullyconstructedpcDNA3-Rzexpressionvectorandlaida basisforfurtherstudyof gene therapy.
作者 严瑞兰 金明 钱新华 惠宏襄 辛晓燕 王健 王检波
出处 《Journal of Medical Colleges of PLA(China)》 CAS 2001年第2期102-106,共5页 中国人民解放军军医大学学报（英文版）
基金 This work is supported by program of National Natural Science Foundation of China(39700147)
关键词 vascularendothelialgrowthfactor hairpinribozyme GENETHERAPY 血管内皮生长因子 核糖酶 基因治疗 肿瘤
分类号 R346 [医药卫生—基础医学]
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参考文献9

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二级参考文献1

  • 1Harris A L，Breast Cancer Res Treat，1996年，38卷，1期，97页

共引文献19

Journal of Medical Colleges of PLA(China)
2001年 第2期

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