摘要
Objective To determine over-expression of a truncated type ⅡTGF-β receptor in down-regulating TGF-β1 auto production in normal dermal fibroblasts. Methods In vitro cultured dermal fibroblasts were treated with rhTGF-β1 (5ng/ml) or recombinant adenovirus containing α truncated type Ⅱ TGF-β receptor gene (50 pfu/cell). Their effects on regulating gene expression of TGF-β1 were observed with Northern Blot. Results rh TGF-β1 up-regulated the gene expression of TGF-β1, (34 %-150%) and type Ⅰ pro-collagen( 13 %- 190%). Overexpression of a truncated receptor Ⅱ decreased the gene expression of TGF-β1 (53%-66%). Conclusion Over-expression of the truncated TGF-β receptor Ⅱdown-regulated TGF-β1 autoproduction via blocking signal transduction of TGF-β. This study may provide a new strategy for scar gene therapy.
Objective:To determine over-expression of a truncated bypeⅡTGF-βreceptor in down-regulating TGF-β1 autoproduction in normal dermal fibroblasts.Methods:In vitro cultured dermal fibro-blasts were treated with rhTGF-β1(5ng/ml)or recombinant adenovirus containing a truncated typeII TGF-βreceptor gene(50 pfu/cell).Their effects on regulating gene expression of TGF-β1 were observed with North-ern Blot.Results:rhTGF-β1 up-regulated the gene expression of TGF-β1(34%-150%)and typeⅠpro-collagen(13%-190%).Over-expression of a truncated receptor Ⅱdecreased the gene expression of TGF-β(53%-66%).Conclusion:Over-expression of the truncated TGF-β receptor Ⅱ down-regulated TGF-β1 autoproduction via blocking signal transduction of TGF-β.This study may provide a new strategy for scar gene therapy.
基金
Supported by Shanghai Science and Technology Development Foundation(00JC14032)
